MYD88 Mouse Monoclonal Antibody [Clone ID: OTI1B4]

CAT#: CF502104

Carrier-free (BSA/glycerol-free) MYD88 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYD88 (RC229151, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYD88. Positive lysates LY432175 (100ug) and LC432175 (20ug) can be purchased separately from OriGene.

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  Anti-MYD88 mouse monoclonal antibody (TA502104) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MYD88 (RC202253).

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  HEK293T cells transfected with either RC202253 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MYD88 antibody (TA502104), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-MYD88 antibody (TA502104), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-MYD88 antibody (TA502104), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1B4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MYD88 (NP_002459) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.1 kDa
• Gene Name: Homo sapiens MYD88 innate immune signal transduction adaptor (MYD88), transcript variant 2, mRNA.
• Database Link:
  NP_002459
  Entrez Gene 4615 Human
  UniProt ID Q99836
• Background: This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: MYD88D

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis, Toll-like receptor signaling pathway