SAP102 (DLG3) Mouse Monoclonal Antibody [Clone ID: OTI5F1]

CAT#: CF502191

Carrier-free (BSA/glycerol-free) DLG3 mouse monoclonal antibody, clone OTI5F1 (formerly 5F1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DLG3 (RC210860, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLG3. Positive lysates LY402837 (100ug) and LC402837 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC210860 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DLG3 antibody (TA502191), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI5F1
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DLG3 (NP_066943) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 90.1 kDa
• Gene Name: Homo sapiens discs large MAGUK scaffold protein 3 (DLG3), transcript variant 1, mRNA.
• Database Link:
  NP_066943
  Entrez Gene 53310 MouseEntrez Gene 58948 RatEntrez Gene 1741 Human
  UniProt ID Q92796
• Background: This gene encodes a member of the membrane-associated guanylate kinase protein family. The encoded protein may play a role in clustering of NMDA receptors at excitatory synapses. It may also negatively regulate cell proliferation through interaction with the C-terminal region of the adenomatosis polyposis coli tumor suppressor protein. Mutations in this gene have been associated with X-linked mental retardation. Alternatively spliced transcript variants have been described. [provided by RefSeq]
• Synonyms: MRX; MRX90; NEDLG; PPP1R82; SAP102; XLMR