Aquaporin 1 (AQP1) Mouse Monoclonal Antibody [Clone ID: OTI2D10]

CAT#: CF502357

Carrier-free (BSA/glycerol-free) AQP1 mouse monoclonal antibody, clone OTI2D10 (formerly 2D10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AQP1 (RC205304, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AQP1. Positive lysates LY403670 (100ug) and LC403670 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-AQP1 monoclonal antibody at 1:200 dilution.

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  Figure from citation: Western Blot of AQP1 protein level by using anti-AQP1 antibody in human B1647 cells. View Citation

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AQP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502357)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AQP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502357)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-AQP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502357)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-AQP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502357)

• Anti-AQP1 mouse monoclonal antibody (TA502357) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AQP1 (RC205304).

• HEK293T cells transfected with either RC205304 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AQP1 antibody (TA502357), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2D10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AQP1 (NP_932766) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.3 kDa
• Gene Name: Homo sapiens aquaporin 1 (Colton blood group) (AQP1), transcript variant 1, mRNA.
• Database Link:
  NP_932766
  Entrez Gene 11826 MouseEntrez Gene 25240 RatEntrez Gene 358 Human
  UniProt ID P29972
• Background: Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: AQP-CHIP; CHIP28; CO

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane