Aquaporin 1 (AQP1) Mouse Monoclonal Antibody [Clone ID: OTI2D10]

CAT#: TA502357S

AQP1 (Aquaporin 1) mouse monoclonal antibody, clone OTI2D10 (formerly 2D10)

Product Images

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human AQP1 plasmid (RC205304, lane 2), mouse AQP1 plasmid (MR203566, lane 3) , rat AQP1 plasmid (RR203590, lane 4) using anti-AQP1 antibody TA502357 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Figure from citation: Western Blot of AQP1 protein level by using anti-AQP1 antibody in human B1647 cells. View Citation

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AQP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AQP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-AQP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-AQP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Anti-AQP1 mouse monoclonal antibody (TA502357) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AQP1 (RC205304).

• HEK293T cells transfected with either RC205304 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AQP1 antibody (TA502357), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2D10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AQP1 (NP_932766) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.47 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.3 kDa
• Gene Name: aquaporin 1 (Colton blood group)
• Database Link:
  NP_932766
  Entrez Gene 358 Human
  UniProt ID P29972
• Background: Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: AQP-CHIP; CHIP28; CO

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane