SERPINB4 Mouse Monoclonal Antibody [Clone ID: OTI4C8]

CAT#: CF502386

Carrier-free (BSA/glycerol-free) SERPINB4 mouse monoclonal antibody, clone OTI4C8 (formerly 4C8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB4 (RC205612, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB4. Positive lysates LY418979 (100ug) and LC418979 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SERPINB4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502386)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SERPINB4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502386)

Specifications

Product Data

• Clone Name: OTI4C8
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SERPINB4 (NP_002965) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.7 kDa
• Gene Name: Homo sapiens serpin family B member 4 (SERPINB4), transcript variant 1, mRNA.
• Database Link:
  NP_002965
  Entrez Gene 6318 Human
  UniProt ID P48594
• Synonyms: LEUPIN; PI11; SCCA-2; SCCA1; SCCA2

Reference Data

• Protein Families: Druggable Genome, Secreted Protein