ACOT12 Mouse Monoclonal Antibody [Clone ID: OTI1B9]

CAT#: CF502462

Carrier-free (BSA/glycerol-free) ACOT12 mouse monoclonal antibody, clone OTI1B9 (formerly 1B9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACOT12 (RC210445, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACOT12. Positive lysates LY408930 (100ug) and LC408930 (20ug) can be purchased separately from OriGene.

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  Flow cytometric Analysis of Hela cells, using anti-ACOT12 antibody (TA502462), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  HEK293T cells transfected with either RC210445 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACOT12 antibody (TA502462), and then analyzed by flow cytometry.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502462)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502462)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502462)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502462)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502462)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502462)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502462)

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Specifications

Product Data

• Clone Name: OTI1B9
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACOT12 (NP_570123) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.9 kDa
• Gene Name: Homo sapiens acyl-CoA thioesterase 12 (ACOT12), mRNA.
• Database Link:
  NP_570123
  Entrez Gene 711665 MonkeyEntrez Gene 134526 Human
  UniProt ID Q8WYK0
• Synonyms: Cach; CACH-1; STARD15; THEAL

Reference Data

• Protein Pathways: Pyruvate metabolism