ARFGAP1 Mouse Monoclonal Antibody [Clone ID: OTI2D5]

CAT#: CF502691

Carrier-free (BSA/glycerol-free) ARFGAP1 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5)

Formulation: Standard Carrier Free


  View other "OTI2D5" antibodies (2)

USD 465.00

4 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI2D5
Applications FC, IF, WB
Recommended Dilution WB 1:500~2000, IF 1:100, FLOW 1:100
Reactivities Human, Mouse, Rat
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Full length human recombinant protein of human ARFGAP1 (NP_783202) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 45.5 kDa
Gene Name Homo sapiens ADP ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2, mRNA.
Background The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Synonyms ARF1GAP; HRIHFB2281
Reference Data
Protein Pathways Endocytosis

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.