ARFGAP1 Mouse Monoclonal Antibody [Clone ID: OTI2D5]

CAT#: CF502691

Carrier-free (BSA/glycerol-free) ARFGAP1 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARFGAP1 (RC206987, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARFGAP1. Positive lysates LY406240 (100ug) and LC406240 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARFGAP1 monoclonal antibody.

• 

  Anti-ARFGAP1 mouse monoclonal antibody (TA502691) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARFGAP1 (RC206987).

• 

  HEK293T cells transfected with either RC206987 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARFGAP1 antibody (TA502691), and then analyzed by flow cytometry.

• 

  Flow cytometric Analysis of Hela cells, using anti-ARFGAP1 antibody (TA502691), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• 

  Flow cytometric Analysis of Jurkat cells, using anti-ARFGAP1 antibody (TA502691), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Show all 6 images

Specifications

Product Data

• Clone Name: OTI2D5
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ARFGAP1 (NP_783202) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.5 kDa
• Gene Name: Homo sapiens ADP ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2, mRNA.
• Database Link:
  NP_783202
  Entrez Gene 228998 MouseEntrez Gene 246310 RatEntrez Gene 55738 Human
  UniProt ID Q8N6T3
• Background: The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: ARF1GAP; HRIHFB2281

Reference Data

• Protein Pathways: Endocytosis