Guanylate kinase (GUK1) Mouse Monoclonal Antibody [Clone ID: OTI4A8]

CAT#: CF502692

Carrier-free (BSA/glycerol-free) GUK1 mouse monoclonal antibody, clone OTI4A8 (formerly 4A8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GUK1 (RC202510, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GUK1. Positive lysates LY400303 (100ug) and LC400303 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GUK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502692)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GUK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502692)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GUK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502692)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GUK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502692)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GUK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502692)

• HEK293T cells transfected with either RC202510 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GUK1 antibody (TA502692), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-GUK1 antibody (TA502692), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-GUK1 antibody (TA502692), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI4A8
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GUK1 (NP_000849) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.5 kDa
• Gene Name: Homo sapiens guanylate kinase 1 (GUK1), transcript variant 2, mRNA.
• Database Link:
  NP_000849
  Entrez Gene 14923 MouseEntrez Gene 303179 RatEntrez Gene 2987 Human
  UniProt ID Q16774
• Synonyms: GMK

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism