RBP1 Mouse Monoclonal Antibody [Clone ID: OTI2H3]

CAT#: CF502755

Carrier-free (BSA/glycerol-free) RBP1 mouse monoclonal antibody, clone OTI2H3 (formerly 2H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBP1 (RC229119, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBP1. Positive lysates LY401017 (100ug) and LC401017 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-RBP1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502755)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502755)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502755)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502755)

• Anti-RBP1 mouse monoclonal antibody (TA502755) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RBP1 (RC214515 ).

• Immunofluorescent staining of HeLa cells using anti-RBP1 mouse monoclonal antibody (TA502755).

• HEK293T cells transfected with either RC214515 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RBP1 antibody (TA502755), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-RBP1 antibody (TA502755), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-RBP1 antibody (TA502755), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI2H3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RBP1 (NP_002890) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15.7 kDa
• Gene Name: Homo sapiens retinol binding protein 1 (RBP1), transcript variant 1, mRNA.
• Database Link:
  NP_002890
  Entrez Gene 19659 MouseEntrez Gene 25056 RatEntrez Gene 5947 Human
  UniProt ID P09455
• Background: This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: CRABP-I; CRBP; CRBP1; CRBPI; RBPC