IKB zeta (NFKBIZ) Mouse Monoclonal Antibody [Clone ID: OTI1E8]

CAT#: CF502768

Carrier-free (BSA/glycerol-free) NFKBIZ mouse monoclonal antibody, clone OTI1E8 (formerly 1E8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NFKBIZ (RC219121, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NFKBIZ. Positive lysates LY410530 (100ug) and LC410530 (20ug) can be purchased separately from OriGene.

• 

  HEK293T cells transfected with either RC219121 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NFKBIZ antibody (TA502768), and then analyzed by flow cytometry.

• 

  Flow cytometric Analysis of Hela cells, using anti-NFKBIZ antibody (TA502768), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• 

  Flow cytometric Analysis of Jurkat cells, using anti-NFKBIZ antibody (TA502768), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI1E8
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NFKBIZ (NP_113607) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77.9 kDa
• Gene Name: Homo sapiens NFKB inhibitor zeta (NFKBIZ), transcript variant 1, mRNA.
• Database Link:
  NP_113607
  Entrez Gene 304005 RatEntrez Gene 64332 Human
  UniProt ID Q9BYH8
• Background: This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: IKBZ; INAP; MAIL

Reference Data

• Protein Families: Druggable Genome