IRF6 Mouse Monoclonal Antibody [Clone ID: OTI2C10]

CAT#: CF503159

Carrier-free (BSA/glycerol-free) IRF6 mouse monoclonal antibody, clone OTI2C10 (formerly 2C10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF6 (RC201579, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF6. Positive lysates LY401852 (100ug) and LC401852 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IRF6 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503159)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503159)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503159)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503159)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503159)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503159)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503159)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503159)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503159)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503159)

• Anti-IRF6 mouse monoclonal antibody (TA503159) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IRF6 (RC201579).

• HEK293T cells transfected with either RC201579 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IRF6 antibody (TA503159), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2C10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IRF6(NP_006138) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.9 kDa
• Gene Name: Homo sapiens interferon regulatory factor 6 (IRF6), transcript variant 1, mRNA.
• Database Link:
  NP_006138
  Entrez Gene 54139 MouseEntrez Gene 364081 RatEntrez Gene 480015 DogEntrez Gene 3664 Human
  UniProt ID O14896
• Background: This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. The encoded protein may be a transcriptional activator. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. Mutations in this gene are also associated with non-syndromic orofacial cleft type 6. Alternate splicing results in multiple transcript variants.
• Synonyms: LPS; OFC6; PIT; PPS; PPS1; VWS; VWS1

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors