IRF6 Mouse Monoclonal Antibody [Clone ID: OTI1D4]

CAT#: CF503338

Carrier-free (BSA/glycerol-free) IRF6 mouse monoclonal antibody, clone OTI1D4 (formerly 1D4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF6 (RC201579, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF6. Positive lysates LY401852 (100ug) and LC401852 (20ug) can be purchased separately from OriGene.

• 

  Anti-IRF6 mouse monoclonal antibody (TA503338) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IRF6 (RC201579).

• 

  HEK293T cells transfected with either RC201579 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IRF6 antibody (TA503338), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1D4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IRF6(NP_006138)produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.9 kDa
• Gene Name: Homo sapiens interferon regulatory factor 6 (IRF6), transcript variant 1, mRNA.
• Database Link:
  NP_006138
  Entrez Gene 54139 MouseEntrez Gene 364081 RatEntrez Gene 3664 Human
  UniProt ID O14896
• Background: This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation. [provided by RefSeq]
• Synonyms: LPS; OFC6; PIT; PPS; PPS1; VWS; VWS1

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors