TMPRSS5 Mouse Monoclonal Antibody [Clone ID: OTI6E11]

CAT#: CF503427

Carrier-free (BSA/glycerol-free) TMPRSS5 mouse monoclonal antibody, clone OTI6E11 (formerly 6E11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMPRSS5 (RC223774, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMPRSS5. Positive lysates LY410720 (100ug) and LC410720 (20ug) can be purchased separately from OriGene.

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  Anti-TMPRSS5 mouse monoclonal antibody (TA503427) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TMPRSS5 (RC223774).

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  HEK293T cells transfected with either RC223774 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TMPRSS5 antibody (TA503427), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI6E11
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TMPRSS5(NP_110397) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.4 kDa
• Gene Name: Homo sapiens transmembrane serine protease 5 (TMPRSS5), transcript variant 1, mRNA.
• Database Link:
  NP_110397
  Entrez Gene 80975 Human
  UniProt ID Q9H3S3
• Background: This gene encodes a protein that belongs to the serine protease family. Serine proteases are known to be involved in many physiological and pathological processes. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: SPINESIN

Reference Data

• Protein Families: Druggable Genome, Protease, Transmembrane