Asparagine synthetase (ASNS) Mouse Monoclonal Antibody [Clone ID: OTI1A10]

CAT#: CF503452

Carrier-free (BSA/glycerol-free) ASNS mouse monoclonal antibody, clone OTI1A10 (formerly 1A10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASNS (RC215380, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASNS. Positive lysates LY403336 (100ug) and LC403336 (20ug) can be purchased separately from OriGene.

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  Anti-ASNS mouse monoclonal antibody (TA503452) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ASNS (RC215380).

Specifications

Product Data

• Clone Name: OTI1A10
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ASNS(NP_597680) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 64.2 kDa
• Gene Name: Homo sapiens asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 1, mRNA.
• Database Link:
  NP_597680
  Entrez Gene 25612 RatEntrez Gene 27053 MouseEntrez Gene 440 Human
  UniProt ID P08243
• Background: The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq]
• Synonyms: ASNSD; TS11

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Nitrogen metabolism