CNN2 Mouse Monoclonal Antibody [Clone ID: OTI2B5]

CAT#: CF503688

Carrier-free (BSA/glycerol-free) CNN2 mouse monoclonal antibody, clone OTI2B5 (formerly 2B5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNN2 (RC221104, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNN2. Positive lysates LY418024 (100ug) and LC418024 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CNN2 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CNN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503688)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CNN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503688)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CNN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503688)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CNN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503688)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CNN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503688)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CNN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503688)

• Anti-CNN2 mouse monoclonal antibody (TA503688) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CNN2 (RC221104).

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Specifications

Product Data

• Clone Name: OTI2B5
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CNN2(NP_004359) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.5 kDa
• Gene Name: Homo sapiens calponin 2 (CNN2), transcript variant 1, mRNA.
• Database Link:
  NP_004359
  Entrez Gene 12798 MouseEntrez Gene 690976 RatEntrez Gene 721164 MonkeyEntrez Gene 1265 Human
  UniProt ID Q99439
• Background: The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: calponin 2; calponin H2; neutral calponin; smooth muscle