ERAS Mouse Monoclonal Antibody [Clone ID: OTI3A3]

CAT#: CF503753

Carrier-free (BSA/glycerol-free) ERAS mouse monoclonal antibody, clone OTI3A3 (formerly 3A3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERAS (RC210965, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERAS. Positive lysates LY405694 (100ug) and LC405694 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3A3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ERAS(NP_853510) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.1 kDa
• Gene Name: Homo sapiens ES cell expressed Ras (ERAS), mRNA.
• Database Link:
  NP_853510
  Entrez Gene 3266 Human
  UniProt ID Q7Z444
• Synonyms: HRAS2; HRASP

Reference Data

• Protein Families: Druggable Genome