WDR4 Mouse Monoclonal Antibody [Clone ID: OTI4G1]

CAT#: CF503815

Carrier-free (BSA/glycerol-free) WDR4 mouse monoclonal antibody, clone OTI4G1 (formerly 4G1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WDR4 (RC217569, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR4. Positive lysates LY403257 (100ug) and LC403257 (20ug) can be purchased separately from OriGene.

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  Anti-WDR4 mouse monoclonal antibody (TA503815) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY WDR4 (RC217569).

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  HEK293T cells transfected with either RC217569 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-WDR4 antibody (TA503815), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4G1
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human WDR4(NP_387510) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.3 kDa
• Gene Name: Homo sapiens WD repeat domain 4 (WDR4), transcript variant 2, mRNA.
• Database Link:
  NP_387510
  Entrez Gene 10785 Human
  UniProt ID P57081
• Background: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]
• Synonyms: TRM82; TRMT82