Nucleobindin 1 (NUCB1) Mouse Monoclonal Antibody [Clone ID: OTI1G3]

CAT#: CF504035

Carrier-free (BSA/glycerol-free) NUCB1 mouse monoclonal antibody, clone OTI1G3 (formerly 1G3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUCB1 (RC201786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1. Positive lysates LY416822 (100ug) and LC416822 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NUCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504035)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NUCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504035)

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  Anti-NUCB1 mouse monoclonal antibody (TA504035) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUCB1 (RC201786).

Specifications

Product Data

• Clone Name: OTI1G3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NUCB1(NP_006175) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.7 kDa
• Gene Name: Homo sapiens nucleobindin 1 (NUCB1), mRNA.
• Database Link:
  NP_006175
  Entrez Gene 84595 RatEntrez Gene 4924 Human
  UniProt ID Q02818
• Background: This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq, Jun 2010]
• Synonyms: CALNUC; NUC