DGKA Mouse Monoclonal Antibody [Clone ID: OTI4A11]

CAT#: CF504047

Carrier-free (BSA/glycerol-free) DGKA mouse monoclonal antibody, clone OTI4A11 (formerly 4A11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DGKA (RC222395, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DGKA. Positive lysates LY400535 (100ug) and LC400535 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DGKA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504047)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DGKA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504047)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DGKA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504047)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DGKA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504047)

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  Flow cytometric Analysis of Jurkat cells, using anti-DGKA antibody (TA504047), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI4A11
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DGKA(NP_001336) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82.5 kDa
• Gene Name: Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 3, mRNA.
• Database Link:
  NP_001336
  Entrez Gene 1606 Human
  UniProt ID P23743
• Background: The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq]
• Synonyms: DAGK; DAGK1; DGK-alpha

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system