Iduronate 2 sulfatase (IDS) Mouse Monoclonal Antibody [Clone ID: OTI3B10]

CAT#: CF504276

Carrier-free (BSA/glycerol-free) IDS mouse monoclonal antibody, clone OTI3B10 (formerly 3B10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDS (RC219187, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDS. Positive lysates LY424863 (100ug) and LC424863 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-IDS monoclonal antibody at 1:200 dilution.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IDS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504276)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IDS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504276)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-IDS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504276)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IDS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504276)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-IDS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504276)

• Anti-IDS mouse monoclonal antibody (TA504276) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDS (RC219187).

• Flow cytometric Analysis of Hela cells, using anti-IDS antibody (TA504276), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-IDS antibody (TA504276), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI3B10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IDS(NP_000193) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.2 kDa
• Gene Name: Homo sapiens iduronate 2-sulfatase (IDS), transcript variant 1, mRNA.
• Database Link:
  NP_000193
  Entrez Gene 15931 MouseEntrez Gene 3423 Human
  UniProt ID P22304
• Background: Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence similarity with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq]
• Synonyms: MPS2; SIDS

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycosaminoglycan degradation, Lysosome, Metabolic pathways