NONO Mouse Monoclonal Antibody [Clone ID: OTI3F8]

CAT#: CF504776

Carrier-free (BSA/glycerol-free) NONO mouse monoclonal antibody,clone OTI3F8

Product Images

Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human NONO plasmid (RC206688, hNONO), mouse NONO plasmid (MR207550, mNONO) using anti-NONO antibody TA504776 (1:5000). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:5000)

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HEK293T cells (WT, Cat# LC810293T) and NONO-Knockout HEK293T cells (KO, Cat# LC841199) were separated by SDS-PAGE and immunoblotted with anti-NONO monoclonal antibody TA504776 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NONO mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NONO mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NONO mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NONO mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NONO mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NONO mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NONO mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NONO mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Anti-NONO mouse monoclonal antibody (TA504776) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NONO (RC206688).

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Specifications

Product Data

• Clone Name: OTI3F8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200~1000, IHC 1:150, IF 1:100
• Reactivities: Human, Dog, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 184-385 of human NONO(NP_031389) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.1 kDa
• Gene Name: non-POU domain containing octamer binding
• Database Link:
  NP_031389
  Entrez Gene 53610 MouseEntrez Gene 317259 RatEntrez Gene 612773 DogEntrez Gene 697263 MonkeyEntrez Gene 4841 Human
  UniProt ID Q15233
• Background: This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]
• Synonyms: MRXS34; NMT55; NRB54; P54; P54NRB; PPP1R114

Reference Data

• Protein Families: Druggable Genome, Transcription Factors