NONO Mouse Monoclonal Antibody [Clone ID: OTI5F1]

CAT#: CF504802

Carrier-free (BSA/glycerol-free) NONO mouse monoclonal antibody, clone OTI5F1 (formerly 5F1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (RC206688, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO. Positive lysates LY402135 (100ug) and LC402135 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-NONO monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HEK293T cells (WT, Cat# LC810293T) and NONO-Knockout HEK293T cells (KO, Cat# LC841199) were separated by SDS-PAGE and immunoblotted with anti-NONO monoclonal antibody TA504802 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504802)

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Specifications

Product Data

• Clone Name: OTI5F1
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:150
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 184-385 of human NONO(NP_031389) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.1 kDa
• Gene Name: Homo sapiens non-POU domain containing octamer binding (NONO), transcript variant 2, mRNA.
• Database Link:
  NP_031389
  Entrez Gene 53610 MouseEntrez Gene 317259 RatEntrez Gene 612773 DogEntrez Gene 697263 MonkeyEntrez Gene 4841 Human
  UniProt ID Q15233
• Background: This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]
• Synonyms: MRXS34; NMT55; NRB54; P54; P54NRB; PPP1R114

Reference Data

• Protein Families: Druggable Genome, Transcription Factors