TSC22D1 Mouse Monoclonal Antibody [Clone ID: OTI1G1]

CAT#: CF505047

Carrier-free (BSA/glycerol-free) TSC22D1 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D1 (RC223210, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D1. Positive lysates LY416882 (100ug) and LC416882 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505047)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505047)

Specifications

Product Data

• Clone Name: OTI1G1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TSC22D1(NP_006013) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15.5 kDa
• Gene Name: Homo sapiens TSC22 domain family member 1 (TSC22D1), transcript variant 2, mRNA.
• Database Link:
  NP_006013
  Entrez Gene 21807 MouseEntrez Gene 498545 RatEntrez Gene 8848 Human
  UniProt ID Q15714
• Background: This gene encodes a member of the TSC22 domain family of leucine zipper transcription factors. The encoded protein is stimulated by transforming growth factor beta, and regulates the transcription of multiple genes including C-type natriuretic peptide. The encoded protein may play a critical role in tumor suppression through the induction of cancer cell apoptosis, and a single nucleotide polymorphism in the promoter of this gene has been associated with diabetic nephropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
• Synonyms: Ptg-2; TGFB1I4; TSC22

Reference Data

• Protein Families: Transcription Factors