RIOK2 Mouse Monoclonal Antibody [Clone ID: OTI3E11]

CAT#: CF505140

Carrier-free (BSA/glycerol-free) RIOK2 mouse monoclonal antibody, clone OTI3E11 (formerly 3E11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIOK2 (RC201484, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIOK2. Positive lysates LY402668 (100ug) and LC402668 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RIOK2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RIOK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505140)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RIOK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505140)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RIOK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505140)

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-RIOK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505140)

• Anti-RIOK2 mouse monoclonal antibody (TA505140) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RIOK2 (RC201484).

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Specifications

Product Data

• Clone Name: OTI3E11
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~4000, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 277-552 of human RIOK2(NP_060813) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.1 kDa
• Gene Name: Homo sapiens RIO kinase 2 (RIOK2), transcript variant 1, mRNA.
• Database Link:
  NP_060813
  Entrez Gene 67045 MouseEntrez Gene 700738 MonkeyEntrez Gene 55781 Human
  UniProt ID Q9BVS4
• Synonyms: RIO2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase