MAGEA4 Mouse Monoclonal Antibody [Clone ID: OTI1F9]

CAT#: CF505362

Carrier-free (BSA/glycerol-free) MAGEA4 mouse monoclonal antibody, clone OTI1F9 (formerly 1F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA4 (RC223991, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEA4. Positive lysates LY423276 (100ug) and LC423276 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-MAGEA4 monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505362)

• 

  Flow cytometric analysis of living NCI-H1299 cells, using anti-MAGEA4 antibody (TA505362, Red), compared to an isotype control (green) (1:100).

• 

  Flow cytometric analysis of living A375 cells, using anti-MAGEA4 antibody (TA505362, Red), compared to an isotype control (green) (1:100).

Show all 5 images

Specifications

Product Data

• Clone Name: OTI1F9
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MAGEA4(NP_001011550) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.7 kDa
• Gene Name: Homo sapiens MAGE family member A4 (MAGEA4), transcript variant 4, mRNA.
• Database Link:
  NP_001011550
  Entrez Gene 4103 Human
  UniProt ID P43358
• Background: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: CT1.4; MAGE-41; MAGE-X2; MAGE4; MAGE4A; MAGE4B