PTGES2 Mouse Monoclonal Antibody [Clone ID: OTI2C3]

CAT#: CF505412

Carrier-free (BSA/glycerol-free) PTGES2 mouse monoclonal antibody, clone OTI2C3 (formerly 2C3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTGES2 (RC221207, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTGES2. Positive lysates LY403045 (100ug) and LC403045 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PTGES2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505412)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PTGES2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505412)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PTGES2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505412)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PTGES2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505412)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PTGES2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505412)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PTGES2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505412)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-PTGES2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505412)

• Anti-PTGES2 mouse monoclonal antibody (TA505412) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTGES2 (RC221207).

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Specifications

Product Data

• Clone Name: OTI2C3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PTGES2(NP_079348) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.8 kDa
• Gene Name: Homo sapiens prostaglandin E synthase 2 (PTGES2), transcript variant 1, mRNA.
• Database Link:
  NP_079348
  Entrez Gene 80142 Human
  UniProt ID Q9H7Z7
• Background: The protein encoded by this gene is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene. [provided by RefSeq]
• Synonyms: C9orf15; GBF-1; GBF1; mPGES-2; PGES2

Reference Data

• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways