IL36 gamma (IL36G) Mouse Monoclonal Antibody [Clone ID: OTI6E4]

CAT#: CF505995

Carrier-free (BSA/glycerol-free) IL1F9 mouse monoclonal antibody, clone OTI6E4 (formerly 6E4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F9 (RC224784, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F9. Positive lysates LY412705 (100ug) and LC412705 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IL1F9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505995)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IL1F9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505995)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IL1F9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505995)

Specifications

Product Data

• Clone Name: OTI6E4
• Applications: IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IL1F9(NP_062564) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.5 kDa
• Gene Name: Homo sapiens interleukin 36 gamma (IL36G), transcript variant 1, mRNA.
• Database Link:
  NP_062564
  Entrez Gene 56300 Human
  UniProt ID Q9NZH8
• Synonyms: IL-1F9; IL-1H1; IL-1RP2; IL1E; IL1F9; IL1H1; IL1RP2

Reference Data

• Protein Families: Druggable Genome, Secreted Protein