Lysozyme (LYZ) Mouse Monoclonal Antibody [Clone ID: OTI1D8]

CAT#: CF506192

Carrier-free (BSA/glycerol-free) LYZ mouse monoclonal antibody, clone OTI1D8 (formerly 1D8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LYZ (RC200730, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LYZ. Positive lysates LY400091 (100ug) and LC400091 (20ug) can be purchased separately from OriGene.

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  Anti-LYZ mouse monoclonal antibody (TA506192) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LYZ (RC200730).

Specifications

Product Data

• Clone Name: OTI1D8
• Applications: IF, WB
• Recommended Dilution: WB 1:4000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LYZ(NP_000230) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.7 kDa
• Gene Name: Homo sapiens lysozyme (LYZ), mRNA.
• Database Link:
  NP_000230
  Entrez Gene 4069 Human
  UniProt ID P61626
• Background: This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the anti-microbial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. Missense mutations in LYZ have been identified in heritable renal amyloidosis. [provided by RefSeq, Jul 2008]
• Synonyms: LYZF1; LZM

Reference Data

• Protein Families: Druggable Genome