ARG2 Mouse Monoclonal Antibody [Clone ID: OTI3G5]

CAT#: CF506751

Carrier-free (BSA/glycerol-free) ARG2 mouse monoclonal antibody, clone OTI3G5 (formerly 3G5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ARG2 (Cat# RC206756, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARG2(Cat# TA506751). Positive lysates LY420091 (100ug) and LC420091 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARG2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Western blot analysis of extracts (10ug) from 3 different mouse tissues by using anti-ARG2 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ARG2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ARG2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ARG2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ARG2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Anti-ARG2 mouse monoclonal antibody (TA506751) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARG2 (RC206756).

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Specifications

Product Data

• Clone Name: OTI3G5
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:400~4000, IHC 1:150, IF 1:100
• Reactivities: Human, Dog, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ARG2(NP_001163) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36 kDa
• Gene Name: arginase 2
• Database Link:
  NP_001163
  Entrez Gene 29215 RatEntrez Gene 480364 DogEntrez Gene 384 Human
  UniProt ID P78540
• Synonyms: arginase; arginase 2; kidney arginase; L-arginine amidinohydrolase; L-arginine ureahydrolase; nonhepatic arginase; type II

Reference Data

• Protein Pathways: Arginine and proline metabolism, Metabolic pathways