Methylmalonyl Coenzyme A mutase (MUT) Mouse Monoclonal Antibody [Clone ID: OTI2A8]

CAT#: CF506883

Carrier-free (BSA/glycerol-free) MUT mouse monoclonal antibody, clone OTI2A8 (formerly 2A8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MUT (RC201972, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MUT. Positive lysates LY400099 (100ug) and LC400099 (20ug) can be purchased separately from OriGene.

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  Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MUT (RC201972) using Anti-MUT mouse monoclonal antibody (TA506883) at 1:100 dilution.

Specifications

Product Data

• Clone Name: OTI2A8
• Applications: IF, WB
• Recommended Dilution: WB 1:1000 -1:4000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MUT(NP_000246) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 79.3 kDa
• Gene Name: Homo sapiens methylmalonyl-CoA mutase (MMUT), mRNA; nuclear gene for mitochondrial product.
• Database Link:
  NP_000246
  Entrez Gene 4594 Human
  UniProt ID P22033
• Background: This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. [provided by RefSeq, Jul 2008]
• Synonyms: MCM

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation