HPRT (HPRT1) Mouse Monoclonal Antibody [Clone ID: OTI2F7]

CAT#: CF506954

Carrier-free (BSA/glycerol-free) HPRT1 mouse monoclonal antibody, clone OTI2F7 (formerly 2F7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPRT1 (RC200462, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPRT1. Positive lysates LY400070 (100ug) and LC400070 (20ug) can be purchased separately from OriGene.

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  Anti-HPRT1 mouse monoclonal antibody (TA506954) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HPRT1 (RC200462).

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  Immunofluorescent staining of HeLa cells using anti-HPRT1 mouse monoclonal antibody (TA506954) at 1:100 dilution.

Specifications

Product Data

• Clone Name: OTI2F7
• Applications: IF, WB
• Recommended Dilution: WB 1:6000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HPRT1(NP_000185) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.4 kDa
• Gene Name: Homo sapiens hypoxanthine phosphoribosyltransferase 1 (HPRT1), mRNA.
• Database Link:
  NP_000185
  Entrez Gene 24465 RatEntrez Gene 3251 Human
  UniProt ID P00492
• Background: The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout. [provided by RefSeq, Jun 2009]
• Synonyms: HGPRT; HPRT

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism