Munc 13 4 (UNC13D) Mouse Monoclonal Antibody [Clone ID: OTI1F4]

CAT#: CF507267

Carrier-free (glycerol/BSA-free) UNC13D mouse monoclonal antibody, clone OTI1F4 (formerly 1F4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UNC13D (RC209141, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UNC13D. Positive lysates LY403710 (100ug) and LC403710 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1F4
• Applications: WB
• Recommended Dilution: WB 1:200~1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UNC13D(NP_954712) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 123.1 kDa
• Gene Name: Homo sapiens unc-13 homolog D (UNC13D), mRNA.
• Database Link:
  NP_954712
  Entrez Gene 70450 MouseEntrez Gene 192177 RatEntrez Gene 201294 Human
  UniProt ID Q70J99
• Background: This gene encodes a protein that is a member of the UNC13 family, containing similar domain structure as other family members but lacking an N-terminal phorbol ester-binding C1 domain present in other Munc13 proteins. The protein appears to play a role in vesicle maturation during exocytosis and is involved in regulation of cytolytic granules secretion. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis type 3, a genetically heterogeneous, rare autosomal recessive disorder. [provided by RefSeq, Jul 2008]
• Synonyms: FHL3; HLH3; HPLH3; Munc13-4