SERPINB1 Mouse Monoclonal Antibody [Clone ID: OTI4B3]

CAT#: CF800076

Carrier-free (BSA/glycerol-free) SERPINB1 mouse monoclonal antibody, clone OTI4B3 (formerly 4B3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB1 (RC202138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB1. Positive lysates LY410744 (100ug) and LC410744 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SERPINB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800076)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800076)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800076)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800076)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800076)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800076)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800076)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800076)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800076)

• HEK293T cells transfected with either RC202138 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SERPINB1 antibody (TA800076), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-SERPINB1 antibody (TA800076), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI4B3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 77-306 of human SERPINB1 (NP_109591) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.6 kDa
• Gene Name: Homo sapiens serpin family B member 1 (SERPINB1), transcript variant 1, mRNA.
• Database Link:
  NP_109591
  Entrez Gene 488191 DogEntrez Gene 1992 Human
  UniProt ID P30740
• Synonyms: EI; ELANH2; HEL-S-27; HEL57; LEI; M; MNEI; NEI; PI-2; PI2

Reference Data

• Protein Families: Druggable Genome