USP48 Mouse Monoclonal Antibody [Clone ID: OTI1F10]

CAT#: CF801869

Carrier-free (BSA/glycerol-free) USP48 mouse monoclonal antibody,clone OTI1F10

Product Images

Western blot analysis of HT29 cell lysate (35ug) by using anti-USP48 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and USP48-Knockout HeLa cells (KO, Cat# LC810390) were separated by SDS-PAGE and immunoblotted with anti-USP48 monoclonal antibody TA801869 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-USP48 mouse monoclonal antibody. (TA801869)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-USP48 mouse monoclonal antibody. (TA801869)

Specifications

Product Data

• Clone Name: OTI1F10
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 361-743 of human USP48 (NP_115612) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens ubiquitin specific peptidase 48 (USP48), transcript variant 1, mRNA.
• Database Link:
  NP_115612
  Entrez Gene 170707 MouseEntrez Gene 362636 RatEntrez Gene 84196 Human
  UniProt ID Q86UV5
• Background: This gene encodes a protein containing domains that associate it with the peptidase family C19, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. Family members function as deubiquitinating enzymes, recognizing and hydrolyzing the peptide bond at the C-terminal glycine of ubiquitin. Enzymes in peptidase family C19 are involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
• Synonyms: RAP1GA1; USP31

Reference Data

• Protein Families: Druggable Genome, Protease, Transmembrane