Villin (VIL1) Mouse Monoclonal Antibody [Clone ID: OTI3B3]

CAT#: CF803236

Carrier-free (BSA/glycerol-free) VIL1 mouse monoclonal antibody, clone OTI3B3 (formerly 3B3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIL1 (RC216340, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIL1. Positive lysates LY416173 (100ug) and LC416173 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-VIL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (TA803236) (1:100)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-VIL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA803236) (1:100)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-VIL1 mouse monoclonal antibody. (TA803236) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-VIL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA803236) (1:100)

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Specifications

Product Data

• Clone Name: OTI3B3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-188 of human VIL1 (NP_009058) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 92.5 kDa
• Gene Name: Homo sapiens villin 1 (VIL1), mRNA.
• Database Link:
  NP_009058
  Entrez Gene 22349 MouseEntrez Gene 316521 RatEntrez Gene 7429 Human
  UniProt ID P09327
• Background: This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]
• Synonyms: D2S1471; VIL