Suppressor of Ty 4 homolog 1 (SUPT4H1) Mouse Monoclonal Antibody [Clone ID: OTI6B10]

CAT#: CF803430

Carrier-free (BSA/glycerol-free) SUPT4H1 mouse monoclonal antibody, clone OTI6B10 (formerly 6B10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUPT4H1 (RC201211, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SUPT4H1. Positive lysates LY418857 (100ug) and LC418857 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6B10
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SUPT4H1 (NP_003159) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 13 kDa
• Gene Name: Homo sapiens SPT4 homolog, DSIF elongation factor subunit (SUPT4H1), transcript variant 1, mRNA.
• Database Link:
  NP_003159
  Entrez Gene 287608 RatEntrez Gene 6827 Human
  UniProt ID P63272
• Synonyms: SPT4; SPT4H; Supt4a; SUPT4H

Reference Data

• Protein Families: Transcription Factors