PPP2R5D Mouse Monoclonal Antibody [Clone ID: OTI5E7]

CAT#: CF803916

Carrier-free (BSA/glycerol-free) PPP2R5D mouse monoclonal antibody, clone OTI5E7 (formerly 5E7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R5D (RC201945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP2R5D. Positive lysates LY416775 (100ug) and LC416775 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PPP2R5D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803916)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PPP2R5D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803916)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PPP2R5D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803916)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PPP2R5D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803916)

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Specifications

Product Data

• Clone Name: OTI5E7
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPP2R5D (NP_006236) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 69.8 kDa
• Gene Name: Homo sapiens protein phosphatase 2 regulatory subunit B'delta (PPP2R5D), transcript variant 1, mRNA.
• Database Link:
  NP_006236
  Entrez Gene 21770 MouseEntrez Gene 363193 RatEntrez Gene 5528 Human
  UniProt ID Q14738
• Synonyms: B56D; MRD35

Reference Data

• Protein Families: Phosphatase
• Protein Pathways: Oocyte meiosis, Wnt signaling pathway