TFB1M Mouse Monoclonal Antibody [Clone ID: OTI6G4]

CAT#: CF804099

Carrier-free (BSA/glycerol-free) TFB1M mouse monoclonal antibody, clone OTI6G4 (formerly 6G4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFB1M (RC204643, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFB1M. Positive lysates LY402490 (100ug) and LC402490 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6G4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 116-346 of human TFB1M (NP_057104) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.4 kDa
• Gene Name: Homo sapiens transcription factor B1, mitochondrial (TFB1M), transcript variant 1, mRNA.
• Database Link:
  NP_057104
  Entrez Gene 308140 RatEntrez Gene 51106 Human
  UniProt ID Q8WVM0
• Synonyms: CGI-75; CGI75; mtTFB; mtTFB1

Reference Data

• Protein Families: Transcription Factors