MYF5 Mouse Monoclonal Antibody [Clone ID: OTI2G5]

CAT#: CF804187

Carrier-free (BSA/glycerol-free) MYF5 mouse monoclonal antibody, clone OTI2G5 (formerly 2G5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYF5 (RC210156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYF5. Positive lysates LY417200 (100ug) and LC417200 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804187)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804187)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804187)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804187)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804187)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804187)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804187)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804187)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804187)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804187)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804187)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804187)

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Specifications

Product Data

• Clone Name: OTI2G5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MYF5 (NP_005584) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.1 kDa
• Gene Name: Homo sapiens myogenic factor 5 (MYF5), mRNA.
• Database Link:
  NP_005584
  Entrez Gene 17877 MouseEntrez Gene 299766 RatEntrez Gene 4617 Human
  UniProt ID P13349
• Synonyms: bHLHc2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors