TFB1M Mouse Monoclonal Antibody [Clone ID: OTI10H8]

CAT#: CF804252

Carrier-free (BSA/glycerol-free) TFB1M mouse monoclonal antibody, clone OTI10H8 (formerly 10H8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFB1M (RC204643, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFB1M. Positive lysates LY402490 (100ug) and LC402490 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI10H8
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 116-346 of human TFB1M (NP_057104) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.4 kDa
• Gene Name: Homo sapiens transcription factor B1, mitochondrial (TFB1M), transcript variant 1, mRNA.
• Database Link:
  NP_057104
  Entrez Gene 308140 RatEntrez Gene 51106 Human
  UniProt ID Q8WVM0
• Background: The protein encoded by this gene is a dimethyltransferase that methylates the conserved stem loop of mitochondrial 12S rRNA. The encoded protein also is part of the basal mitochondrial transcription complex and is necessary for mitochondrial gene expression. The methylation and transcriptional activities of this protein are independent of one another. Variations in this gene may influence the severity of aminoglycoside-induced deafness (AID). [provided by RefSeq, Aug 2010]
• Synonyms: CGI-75; CGI75; mtTFB; mtTFB1

Reference Data

• Protein Families: Transcription Factors