ID4 Mouse Monoclonal Antibody [Clone ID: OTI3H1]

CAT#: CF804983

Carrier-free (BSA/glycerol-free) ID4 mouse monoclonal antibody, clone OTI3H1 (formerly 3H1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID4 (RC204170, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID4. Positive lysates LY419876 (100ug) and LC419876 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3H1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ID4 (NP_001537) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.4 kDa
• Gene Name: Homo sapiens inhibitor of DNA binding 4, HLH protein (ID4), mRNA.
• Database Link:
  NP_001537
  Entrez Gene 15904 MouseEntrez Gene 291023 RatEntrez Gene 3400 Human
  UniProt ID P47928
• Background: This gene encodes a member of the inhibitor of DNA binding (ID) protein family. These proteins are basic helix-loop-helix transcription factors which can act as tumor suppressors but lack DNA binding activity. Consequently, the activity of the encoded protein depends on the protein binding partner. [provided by RefSeq, Dec 2011]
• Synonyms: bHLHb27; IDB4

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway