PLAT Mouse Monoclonal Antibody [Clone ID: OTI3H3]

CAT#: CF805448

Carrier-free (BSA/glycerol-free) PLAT mouse monoclonal antibody, clone OTI3H3 (formerly 3H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLAT (RC210571, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLAT. Positive lysates LY400338 (100ug) and LC400338 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PLAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805448)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PLAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805448)

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-PLAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805448)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PLAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805448)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PLAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805448)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PLAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805448)

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Specifications

Product Data

• Clone Name: OTI3H3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 157-426 of human PLAT(NP_000921) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60.5 kDa
• Gene Name: Homo sapiens plasminogen activator, tissue type (PLAT), transcript variant 1, mRNA.
• Database Link:
  NP_000921
  Entrez Gene 5327 Human
  UniProt ID P00750
• Background: This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
• Synonyms: T-PA; TPA

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades