GFI1 Mouse Monoclonal Antibody [Clone ID: OTI6E8]

CAT#: CF805544

Carrier-free (glycerol/BSA-free) GFI1 mouse monoclonal antibody, clone OTI6E8 (formerly 6E8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFI1 (RC207574, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFI1. Positive lysates LY417416 (100ug) and LC417416 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GFI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805544)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GFI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805544)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GFI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805544)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GFI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805544)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GFI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805544)

Show all 6 images

Specifications

Product Data

• Clone Name: OTI6E8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-180 of human GFI1(NP_005254) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.1 kDa
• Gene Name: Homo sapiens growth factor independent 1 transcriptional repressor (GFI1), transcript variant 1, mRNA.
• Database Link:
  NP_005254
  Entrez Gene 14581 MouseEntrez Gene 24388 RatEntrez Gene 2672 Human
  UniProt ID Q99684
• Background: This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: GFI-1; GFI1A; SCN2; ZNF163

Reference Data

• Protein Families: Druggable Genome, Transcription Factors