GFI1 Mouse Monoclonal Antibody [Clone ID: OTI4E2]

CAT#: CF806041

Carrier-free (BSA/glycerol-free) GFI1 mouse monoclonal antibody, clone OTI4E2 (formerly 4E2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFI1 (RC207574, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFI1. Positive lysates LY417416 (100ug) and LC417416 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4E2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-180 of human GFI1(NP_005254) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens growth factor independent 1 transcriptional repressor (GFI1), transcript variant 1, mRNA.
• Database Link:
  NP_005254
  Entrez Gene 14581 MouseEntrez Gene 24388 RatEntrez Gene 2672 Human
  UniProt ID Q99684
• Synonyms: GFI-1; GFI1A; SCN2; ZNF163

Reference Data

• Protein Families: Druggable Genome, Transcription Factors