Lipin 1 (LPIN1) Mouse Monoclonal Antibody [Clone ID: OTI5B5]

CAT#: CF806467

Carrier-free (BSA/glycerol-free) LPIN1 mouse monoclonal antibody, clone OTI5B5 (formerly 5B5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LPIN1 (RC207138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LPIN1. Positive lysates LY407897 (100ug) and LC407897 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LPIN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806467) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-LPIN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806467) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LPIN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806467) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LPIN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806467) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LPIN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806467) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-LPIN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806467) Dilution: 1:150

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Specifications

Product Data

• Clone Name: OTI5B5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-274 of human LPIN1(NP_663731) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens lipin 1 (LPIN1), transcript variant 1, mRNA.
• Database Link:
  NP_663731
  Entrez Gene 14245 MouseEntrez Gene 313977 RatEntrez Gene 23175 Human
  UniProt ID Q14693
• Synonyms: PAP1