ROR1 Mouse Monoclonal Antibody [Clone ID: OTI3D11]

CAT#: CF806826

Carrier-free (BSA/glycerol-free) ROR1 mouse monoclonal antibody, clone OTI3D11 (formerly 3D11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROR1 (RC214967, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ROR1. Positive lysates LY401558 (100ug) and LC401558 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3D11
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ROR1 (NP_005003) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 104.1 kDa
• Gene Name: Homo sapiens receptor tyrosine kinase like orphan receptor 1 (ROR1), transcript variant 1, mRNA.
• Database Link:
  NP_005003
  Entrez Gene 26563 MouseEntrez Gene 362550 RatEntrez Gene 4919 Human
  UniProt ID Q01973
• Background: This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun
• Synonyms: dJ537F10.1; NTRKR1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane