Granulin (GRN) Mouse Monoclonal Antibody [Clone ID: OTI2D1]

CAT#: CF807357

Carrier-free (BSA/glycerol-free) GRN mouse monoclonal antibody,clone OTI2D1

Product Images

Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-GRN monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and GRN-Knockout 293T cells (KO, Cat# LC811733) were separated by SDS-PAGE and immunoblotted with anti-GRN monoclonal antibody TA807357, (1:100). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807357) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807357) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA (1:500)

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  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA (1:0) (1:500)

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Specifications

Product Data

• Clone Name: OTI2D1
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 327-593 of human GRN(NP_002078) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.54 kDa
• Gene Name: Homo sapiens granulin precursor (GRN), mRNA.
• Database Link:
  NP_002078
  Entrez Gene 2896 Human
  UniProt ID P28799
• Synonyms: CLN11; GEP; GP88; PCDGF; PEPI; PGRN

Reference Data

• Protein Families: Druggable Genome, Secreted Protein