Islet 1 (ISL1) Mouse Monoclonal Antibody [Clone ID: OTI6E8]

CAT#: CF807583

Carrier-free (BSA/glycerol-free) ISL1 mouse monoclonal antibody,clone OTI6E8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ISL1 (RC206893, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ISL1 (1:2000). Positive lysates LY400801 (100ug) and LC400801 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6E8
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 134-349 of human ISL1(NP_002193) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.9 kDa
• Gene Name: Homo sapiens ISL LIM homeobox 1 (ISL1), mRNA.
• Database Link:
  NP_002193
  Entrez Gene 16392 MouseEntrez Gene 64444 RatEntrez Gene 3670 Human
  UniProt ID P61371
• Background: This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008]
• Synonyms: Isl-1; ISLET1

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors