ATF3 Mouse Monoclonal Antibody [Clone ID: OTI5G9]

CAT#: CF808558

Carrier-free (BSA/glycerol-free) ATF3 mouse monoclonal antibody, clone OTI5G9 (formerly 5G9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATF3 (RC202897, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATF3 (1:2000). Positive lysates LY422248 (100ug) and LC422248 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5G9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ATF3(NP_001025458) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.4 kDa
• Gene Name: Homo sapiens activating transcription factor 3 (ATF3), transcript variant 3, mRNA.
• Database Link:
  NP_001025458
  Entrez Gene 11910 MouseEntrez Gene 25389 RatEntrez Gene 467 Human
  UniProt ID P18847
• Background: This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]
• Synonyms: FLJ41705

Reference Data

• Protein Families: Transcription Factors