KRT23 Mouse Monoclonal Antibody [Clone ID: OTI3F2]

CAT#: CF808818

Carrier-free (BSA/glycerol-free) KRT23 mouse monoclonal antibody, clone OTI3F2 (formerly 3F2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT23 (RC205221, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT23 (1:2000). Positive lysates LY402445 (100ug) and LC402445 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3F2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-270 of human KRT23(NP_056330) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48 kDa
• Gene Name: Homo sapiens keratin 23 (KRT23), transcript variant 1, mRNA.
• Database Link:
  NP_056330
  Entrez Gene 25984 Human
  UniProt ID Q9C075
• Background: The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
• Synonyms: CK23; HAIK1; K23